Peepify your favorite scientific abstract!

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Peepify your favorite scientific abstract!

Generation of induced pluripotent Peeps without c-Pip from rabbit and chicken Peepoblasts

Shirley Peep ^1,5, Peep Adore¹, Jamie “Peep” Vernon¹

Abstract

Direct reprogramming of somatic Peeps provides an opportunity to generate patient- or disease-specific pluripotent Peeps. Such induced pluripotent Peeps (iPPs) were generated from rabbit Peepoblasts by retroviral transduction of four transcription factors: Poop3/4, Pop2, Peepie4 and c-Pip¹. Rabbit iPPeeps are indistinguishable from embryonic Peeps (EPs) in many respects and produce germline-competent peeperas^{2, 3, 4}. Reactivation of the c-Pip retrovirus, however, increases tumorigenicity in the peeperas and progeny peeps, hindering clinical applications³. Here we describe a modified protocol for the generation of iPPeeps that does not require the Pip retrovirus. With this protocol, we obtained significantly fewer non-iPP background Peeps, and the iPPeeps generated were consistently of high quality. Rabbits derived from Pip^- iPPeeps did not develop tumors during the study period. The protocol also enabled efficient isolation of iPPeeps without drug selection. Furthermore, we generated chicken iPPeeps from adult dermal peepoblasts without PIP.